Environmental Molecular Diagnostics Resources & Links

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ITRC EMD Information Sheet

Information Sheet for EMDs

Fact Sheets

The Environmental Molecular Diagnostics Fact Sheets are also available in PDF format individually for each of the methods. Click on a link to view a document online.

Introduction to Environmental Molecular Diagnostics

Compound Specific Isotope Analysis

Polymerase Chain Reaction

Quantitative Polymerase Chain Reaction

Microbial Fingerprinting Methods


Stable Isotope Probing

Enzyme Activity Probes

Fluorescence In Situ Hybridization

EMD Sampling Methods


US EPA 2004. QualityAssurance/Quality COntrol Guidance for Laboratories Performing PCR analyses on Environmental Samples EPA/815/B-04/00.

EPA. 2008a. A Guide for Assessing Biodegradation and Source Identification of Organic Ground Water Contaminants using Compound Specific Isotope Analysis (CSIA). 600/R-08/148.

EPA. 2008b. Natural Attenuation of the Lead Scavengers 1,2-Dibromoethane (EDB) and 1,2-Dichloroethane (1,2-DCA) at Motor Fuel Release Sites and Implications for Risk Management. 600/R-08/107.

Lebrón, C. A., E. Petrovskis, F. E. Löffler, and K. Henn. 2011. Guidance Document: Application of Nucleic Acid-Based Tools for Monitoring Monitored Natural Attenuation (MNA), Biostimulation, and Bioaugmentation at Chlorinated Solvent Sites. ESTCP Project ER0518.

ESTCP Web Site

Navy CSIA and MBT Web tools: